Mastering Biochemistry for NEET PG 2026: The Ultimate High-Yield Roadmap

• Chromatin structure
• Nucleotides
• De novo pyrimidine and purine synthesis
• Purine salvage deficiencies
• Genetic code features
• DNA replication
• DNA repair
• Mutations in DNA
• Lac operon
• Functional organization of a eukaryotic gene
• Regulation of gene expression
• RNA processing (eukaryotes)
• RNA polymerases
• Introns vs exons
• Splicing of pre-mRNA
• tRNA
• Start and stop codons
• Protein synthesis
• Posttranslational modifications
• Chaperone protein
• Cell cycle phases
• Rough endoplasmic reticulum
• Smooth endoplasmic reticulum
• Cell trafficking
• Peroxisome
• Proteasome
• Cytoskeletal elements
• Microtubule
• Cilia structure
• Primary ciliary dyskinesia
• Sodium-potassium pump
• Collagen
• Collagen synthesis and structure
• Osteogenesis imperfecta
• Ehlers-Danlos syndrome
• Menkes disease
• Elastin
• Marfan syndrome
• Homocystinuria
• Polymerase chain reaction
• CRISPR/Cas9
• Blotting procedures
• Flow cytometry
• Microarrays
• Enzyme-linked immunosorbent assay
• Karyotyping
• Fluorescence in situ hybridization
• Molecular cloning
• Gene expression modifications
• Genetic terms
• Population genetics
• Hardy-Weinberg principle
• Disorders of imprinting
• Modes of inheritance
• Autosomal dominant diseases
• Autosomal recessive diseases
• Cystic fibrosis
• X-linked recessive diseases
• Muscular dystrophies
• Mitochondrial diseases
• Rett syndrome
• Fragile X syndrome
• Trinucleotide repeat expansion diseases
• Autosomal trisomies
• Genetic disorders by chromosome
• Robertsonian translocation
• Cri-du-chat syndrome
• Williams syndrome
• Essential fatty acids
• Vitamins: fat soluble
• Vitamins: water soluble
• Dietary supplementation
• Vitamin A
• Vitamin B1
• Vitamin B2
• Vitamin B3
• Vitamin B5
• Vitamin B6
• Vitamin B7
• Vitamin B9
• Vitamin B12
• Vitamin C
• Vitamin D
• Vitamin E
• Vitamin K
• Zinc
• Protein-energy malnutrition
• Ethanol metabolism
• Enzyme terminology
• Rate-determining enzymes of metabolic processes
• Metabolic compartmentation
• Summary of pathways
• Activated carriers
• Universal electron acceptors
• Hexokinase vs glucokinase
• Glycolysis regulation
• Regulation by fructose-2,6- bisphosphate
• Pyruvate dehydrogenase complex
• Pyruvate dehydrogenase complex deficiency
• Pyruvate metabolism
• TCA cycle
• Electron transport chain and oxidative phosphorylation
• Gluconeogenesis, irreversible enzymes
• Pentose phosphate pathway
• Glucose-6-phosphate dehydrogenase deficiency
• Disorders of fructose metabolism
• Disorders of galactose metabolism
• Sorbitol
• Lactase deficiency
• Amino acids
• Urea cycle
• Transport of ammonia by alanine
• Hyperammonemia
• Ornithine transcarbamylase deficiency
• Amino acid derivatives
• Catecholamine synthesis/tyrosine catabolism
• Phenylketonuria
• Maple syrup urine disease
• Alkaptonuria
• Homocystinuria
• Cystinuria
• Organic acidemias
• Glycogen regulation by insulin and glucagon/epinephrine
• Glycogen
• Glycogen storage diseases
• Lysosomal storage diseases
• Fatty acid metabolism
• Ketone bodies
• Fasted vs fed state
• Metabolic fuel use
• Fasting and starvation
• Lipid transport
• Key enzymes in lipid transport
• Major apolipoproteins
• Lipoprotein functions
• Abetalipoproteinemia
• Familial dyslipidemias

The Strategic Approach: Why Biochemistry Matters

Instead of treating Biochemistry as a collection of isolated facts, view it as the “molecular language” of medicine. When you understand the biochemical defect, the clinical presentation becomes logical rather than a set of symptoms to memorize.

1. Focus on Clinical Correlations

Questions are increasingly case-based. Don’t just memorize the enzyme deficiency—understand the resulting metabolic block and the patient’s symptoms.

• Pro-tip: When studying a pathway, always ask, “What happens if this enzyme is absent?” and “Which clinical condition mimics this?”

2. Leverage High-Yield Tools

You don’t need to reinvent the wheel. Utilize structured resources to maximize your retention:

• Visual Learning: Use flowcharts for complex pathways like the TCA cycle or the Urea cycle.
• Active Recall: Avoid passive reading. Use mymedschool.org to test yourself with high-yield practice questions immediately after completing a topic.
• The “Rule of 5”: When summarizing notes, group information into no more than five categories per subtopic to prevent cognitive overload.

3 Tips for Effective Revision

1. Iterative Learning: Your brain needs time to consolidate information. Study in 30-minute high-focus bursts followed by short reflections rather than long, multi-hour marathons.
2. Build Your “Go-To” Journal: Instead of just highlighting your textbooks, create a personal notebook with your own mnemonics, enzyme-deficiency tables, and common PYQ pitfalls. This will be your most valuable asset in the final month before the exam.
3. Analyze Trends: Treat previous year questions (PYQs) as your diagnostic tool. If you repeatedly get questions wrong in a specific area (e.g., lipid metabolism), go back to that chapter and prioritize it in your next review cycle.

Final Note: Biochemistry isn’t just about passing the exam; it’s about building the clinical intuition that will serve you throughout your residency. Stay consistent, stay clinical, and utilize the right tools to turn “must-learn” into “already known.”