Competitive inhibition

 

Competitive Inhibition: Kinetic Impact

Parameter Change in Competitive Inhibition
Vmax Unchanged (Can be overcome by high substrate concentrations).
Km Increases (Apparent affinity decreases; more substrate is required).
High-Yield Core Realities:

  • Binding Mechanism: Inhibitor mimics the substrate and binds to the active site.
  • Lineweaver-Burk Plot: Y-intercept (1/Vmax) is unchanged; X-intercept (-1/Km) moves closer to zero.
  • Clinical Examples: Statins (inhibiting HMG-CoA reductase), Fomepizole (inhibiting alcohol dehydrogenase).